A 4-layer PCB with a solid ground plane and a separate analog ground plane is recommended. Keep the analog and digital grounds separate and connect them at a single point. Use a star topology for the power supply connections and decouple the power supplies with 10uF and 100nF capacitors.
Use a low-noise power supply, keep the analog and digital circuits separate, and use a quiet analog ground plane. Use a common-mode filter on the ECG leads and a driven right leg (DRL) circuit to reduce common-mode noise. Also, use a low-pass filter to remove high-frequency noise.
Perform a system calibration by applying a known voltage to the input and measuring the output. Use a calibration coefficient to correct for any gain and offset errors. Perform a calibration at multiple temperatures to account for temperature drift.
Use a shielded enclosure and keep the analog and digital circuits separate. Use a common-mode filter on the ECG leads and a shielded cable for the ECG electrodes. Ensure that the power supply is EMC-compliant and use a ferrite bead or a common-mode choke to filter the power supply lines.
Use a logic analyzer or an oscilloscope to monitor the digital signals. Use a protocol analyzer to debug the SPI interface. Use a voltage meter to measure the analog signals and a current probe to measure the current consumption.
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